Sites of Synthesis of Encephalomyocarditis Virus Components in Infected L-cells.

Selective inhibition of protein synthesis in virus-infected mammalian cells.

A number of translation inhibitors were tested for their effects on both control and encephalomyocarditis virus-infected mouse 3T6 cells. The virus-infected cells were specifically inhibited by gougerotin, edeine, and blasticidin S, whereas these drugs failed to penetrate into uninfected cells. Inhibition of infected cells by gougerotin became apparent when the synthesis of viral proteins comme...

Macromolecular synthesis in cells infected with Herpes simplex virus

Action of human lymphoblastoid interferon on HeLa cells infected with RNA-containing animal viruses.

Protein synthesis in interferon-treated HeLa cells infected by different animal RNA viruses has been studied. Synthesis of vesicular stomatitis virus (VSV) proteins was not detected in cells treated with concentrations of HuIFN-alpha (Ly) above 10 IU/ml. No specific inhibition of glycosylation of the G protein was observed. In addition, inhibition of host protein synthesis in IFN-treated cells ...

Encephalomyocarditis Virus Ribonucleic Acid Polymerase Associated with 150S

A specific cytoplasmic structure with a sedimentation value of about 200S appears to be associated with synthesis and assembly of poliovirus components (13). Girard et al. (8) presented evidence suggesting that polio ribonuleic acid (RNA) polymerase and singleand doublestranded viral RNA from the cytoplasm of infected cells sediment together as 200S virus-synthesizing complexes. We have isolate...

Poly (A) polymerase activity in L cells following encephalomyocarditis virus infection.

Poly (A) polymerase activity has been measured in crude cytoplasmic extracts of mouse L cells infected with encephalomyocarditis (EMC) virus. After infection there is first a decrease in enzyme activity followed by an increase which itself precedes detectable virus RNA and protein synthesis. The activity of the enzyme then declines before the release of mature virions and cell death take place....